Age-related responses to injury and repair in insect cuticle

Abstract

We evaluated the ability of female adult desert locusts (Schistocerca gregaria) to repair injuries to their exoskeletons and restore mechanical strength over the course of their natural life. We discovered that younger insects are more capable of repairing injuries, displaying no significant decreases in failure strength, stiffness or bending moment to failure after 3 weeks of repair. Older insects, in contrast, were only capable of repairing to ∼70% of their original strength. Both older and younger insects carry out targeted deposition to repair injuries. We also examined different mechanisms of failure, and we discovered that the cuticle of older insects is more susceptible to crack growth due to a large decrease in fracture toughness with age, making them more sensitive to scalpel cuts and punctures. The biological mechanisms that drive these changes are still under investigation.