Impact of CYLD on chromatin structure and histone methylation in malignant melanoma

Abstract

The tumor suppressive role of CYLD lysine 63 deubiquitinase (CYLD) is known in melanoma. To the best of our knowledge, however, the precise mechanism under- lying the tumor suppressive function of CYLD has yet to be clarified. In the present study, a novel melanoma mouse model was generated, which revealed accelerated tumor growth in Cyld-knockout (Cyld-/-) compared with Cyld-wild-type (Cyld+/+) mice. To determine the underlying molecular mechanism, mutation analysis of primary tumor-derived cell lines from Cyld+/+and Cyld-/-mice was performed using RNA sequencing data. Variant calling revealed no common mutations in Cyld-/-compared with Cyld+/+cells. Thus, the epigenetic processes influencing development and progression of melanoma were investigated. Initial analysis of expres- sion pattern of known hypermethylated genes in melanoma (suppressor of cytokine signalling, methylthioadenosine phosphorylase, cadherin 1) in the presence or absence of 5'-Aza-deoxyctidine treatment revealed that CYLD does not play a key role in DNA methylation. Chromatin accessibility and histone H3 modification assay uncovered a role of CYLD in the formation of chromatin structure. Subsequent inhibitor experiments confirmed the effect of CYLD on H3K9me2 level associated with heterochromatin. Furthermore, enhanced H3K9 dimethylation in Cyld-/-melanoma cells was associated with upregulation of euchromatic histone lysine methyltrans- ferase 2 (EHMT2). Moreover, the specific inhibitor of EHMT2, CM272, resulted in decreased proliferation and relaxation of compact chromatin in Cyld-deficient melanoma cells. These results reveal a novel role of CYLD in histone methylation and chromatin packaging.