Development and in vitro Evaluation of Nanoemulsion-based Polyherbal Hair Serum Formulations for Leave-in Hair Treatments

Abstract

BackgroundHair is a prominent physical feature that is found in all mammals. Different hair types will display different hair physicochemical properties (colour and appearance), mechanical resistance and reactivity to chemical agents and surface properties.ObjectivesThis study aims to prepare polyherbal-based hair serum to conduct stability tests and in-vitro evaluation tests to assess the efficacy of hair serum on different types of human hair tresses for the purpose of leave-in hair treatments.Methods and MaterialThe hair care formulations, such as hair serum, were prepared and their stability was evaluated through a series of tests. The stability tests were conducted by evaluating the organoleptic properties of the formulations, such as colour, odour, surface appearance, consistency, and clarity, under various environmental conditions over a period of four weeks.ResultsThe relative viscosity of the hair serum is 7.84 mPa/s, relative density is 0.997 g/ml, and an average particle size is 1079 ± 21.22 µm. Over the course of 4 weeks, the nanoemulsion-based hair serum was stable between 25 – 40°C and away from direct sunlight exposure.ConclusionThe formulated polyherbal hair serum can be used as a leave-in treatment to maintain hair’s moisture balance while improving hair combability. Further improvements in the hair product formulation can be made in the future to increase their potential for commercialisation.