Conserved distal loop residues in the HSP104 and CLPB middle domain contact nucleotide-binding domain 2 and enable Hsp70-dependent protein disaggregation

Abstract

Background: How Hsp104 and ClpB coordinate polypeptide handover with Hsp70 to dissolve disordered protein aggregates is unknown. Results: Conserved distal loop residues in the Hsp104 and ClpB middle domain contact NBD2 and enable Hsp70-dependent protein disaggregation. Conclusion: Distal loop does not project out into solution and Hsp104 and ClpB are tuned differently for Hsp70 collaboration. © 2014 by the American Society for Biochemistry and Molecular Biology, Inc. .