LCMS-QTOF Determination of Lentinan-Like β -D-Glucan Content Isolated by Hot Water and Alkaline Solution from Tiger’s Milk Mushroom, Termite Mushroom, and Selected Local Market Mushrooms

Abstract

Lentinan, 1152 Dalton β -D-glucan found in Shiitake Mushroom ( Lentinus edodes ), has been claimed to have anticancer and immunomodulatory activity. Several extraction methods have been used by researchers to isolate Lentinan including hot water and alkaline solution (1.25 M NaOH). In this study, hot water and alkaline solution (1.25 M NaOH) were used to extract the Lentinan-like β -D-glucan (1151 Dalton) from Tiger’s Milk Mushroom, Termite Mushroom, and selected local market mushrooms. The isolated Lentinan-like β -D-glucan from both hot water and alkaline solution was analyzed by LCMS-QTOF. Commercial Lentinan standard from Lentinus edodes was used as a reference. The results showed significant differences on chromatogram patterns of Lentinan-like β -D-glucan between both extracts. The peak of Lentinan-like β -D-glucan was only found in isolated polysaccharide glucan of hot water extracts. The isolated polysaccharide glucans from Tiger’s Milk Mushroom and Termite Mushroom were found to have 0.74 ± 0.12 μ g/mg and 0.53 ± 0.07 μ g/mg Lentinan-like β -D-glucan. Button Mushroom, Shiitake Mushroom, and Oyster Mushroom showed the presence of Lentinan-like β -D-glucan at 16.16 ± 4.15 μ g/mg, 0.22 ± 0.04 , and 0.10 ± 0.01 μ g/mg, respectively.