DDT and Its Metabolites in Fresh Water Fish Samples

Abstract

Twenty two fresh water fish samples namely Puntius sarana (Shawrputi), Cyprinus carpio (Karp), Oreochromis niloticos (Telapia), Channa punctatus (Taki), Wallogonia attu (Boal), Eutropiichthys vacha (Bacha), Macrognathus aculiatus (Baim), Ailia coila (Kajoli), Mystus cavasisus(Gulsa), Ompok pabda (Pabdha), Corica soborna (Kachki), Mystus vittatus (Tengra), Glossogobius giuris (Baila), Macrobrachium malcolmsli (Chingri), Amblypharyngodon microlepis (Mola), Anabas testudineus (Koi), Macrognathus aculiatus (Baim), Channa striatus (Shole), Heteropnueste fossilis (Shing), Puntius sophore (Small Puti) and Pseudambassis ranga (Telapia) were collected from two rivers and one cultured fish pond. The samples were extracted by QuEChERS method, cleaned up with conc. H2SO4 treatment and cleaned extracts were analyzed by GC-ECD. Small size cultured rui fish sample which did show detectable amount of DDT and its metabolites was used for the recovery experiments. Percent recovery was found to be in the range of 70%-113%. Amount of total DDTs were found to be 54.34, 48.81, 62.09, 54.72,78.81, 60.07, 47.0, 42.7, 26.31, 10.36, 25.32, 12.96, 20.10, 12.78, 17.65, and 4.71, 8.58, 11.3 and 19.01 ng/g in gulsa, pabhda, baila, bacha, baim, small puti, tengra, chanda, kachki, boal, taki, chingri, mola, shole, shing, koi, swarputi, karp and telapia fish samples, respectively. However, the residual amounts of DDTs in all the fish samples were below maximum residue limit (MRL of DDTs in fish 5.0 mg/kg) according to Codex Alimentarius Commission. The ratios of 4,4´-DDT/DDTs were in the range of 0.03-0.44 which indicated that exposure to DDT is not due to recent use. Key