Protective effect of guava supplementation in periodontitis rats model

Abstract

This study aimed to investigate the effect of guava supplementation on acute phase periodontitis rats model. Periodontitis was induced by bacterial-Adhered ligature placement around the mandibular incisor tooth. Twenty-four male adult of Sprague Dawley rats were divided into four groups (n=6/group). Sham operated (C) and positive controls (M=metronidazole 45mg/kg, p.o) were included. The rats were treated with ethanolic guava fruits extract (Gp=Gc=135mg/kg, p.o), 7-days before (Gp) and after induction (Gc) of periodontal inflammation. After 14 days of post-induction treatment, the animals were sacrificed. The clinical parameter was gained through measuring the periodontal pocket depth (PPD) using the Williams probe at mesial and lingual sites of rat's mandibular incisors teeth. Samples representing the periodontal tissue were stored in formalin and prepared for histological processing. Induction by ligature placement caused a significant inflammation on periodontal tissue. Tissue repairment was seen clinically in the M and Gp groups with PPD score of lingual, p=0.012 and 0.046, respectively. Histologically, fibrocollagen regeneration was shown to be significantly different only by M group among the other groups. The result show that pre-Treatment with guava extract on Gp group have a positive impact in detaining clinical attachment-loss, better than the post induction-Treatment group at the equal dose (p<0,05).