First Isolation and Identification of Listeria monocytogenes Isolated from Frozen and Shock Frozen Dressed Broiler Chicken in Sudan

Abstract

Aim: To isolate and identify Listeria monocytogenes from frozen and shock frozen raw dressed broiler chicken in Khartoum State, Sudan. Place and Duration of Study: Methodology: Eight hundred samples were used in this the study. Five hundred frozen (-18º) raw dressed broiler chickens samples were collected from five station chicken abattoirs in Khartoum State. Three hundred samples were collected as fresh,-18ºC and shock frozen (-40ºC) raw dressed broiler chickens. Detection and isolation of L. monocytogenes was carried out using the conventional International Organization for Standardization method. Results: Out of the 500 samples, 195(39%) were found to be contaminated with Iisteria spp.; L. monocytogenes 64(12.8%), Listeria ivanovii 97(19.4%), Listeria grayi 20(4%), Listeria seeligeri 5(1%), Listeria welshimeri 9(1.8%). 29 were found to be contaminated with Iisteria spp.; L. monocytogenes 39(13%), Listeria ivanovii 54(18%), Listeria grayi 11(3.6%), Listeria seeligeri 3(1%) and Listeria welshimeri 4(1.3%). Conclusion: The results presented in this study indicated that L. monocytogenes was found in frozen (-18ºC) raw dressed broiler chicken and shock frozen (-40ºC) raw dressed broiler chickens.