A REVIEW OF THE DEVELOPMENT AND APPLICATION OF PHYSICAL AND CHEMICAL PRINCIPLES IN THE CULTIVATION OF OBLIGATELY ANAEROBIC BACTERIA

Abstract

The older methods of cultivating obligate anaerobes have been reviewed by Roux (1887), Novy (1893), Hunziker (1902), Mat- zuschita (1902) and Von Oettingen (1903). The work of Hun- ziker was especially valuable in its time but since then many important improvements in technic have been made. The numerous methods that have been suggested for culti- vating the obligately anaerobic bacteria involve, as a rule, a combination of one or more of the principles listed below under (1) reduction of oxygen tension, with one or more of those listed under (2) maintenance of reduced oxygen tension.