Differences in tomato seed protein profiles obtained by SDS-PAGE analysis

Abstract

The protein profiles of tomato seeds from sub-species ( subsp. cultum Brezh., subsp. subspontaneum Brezh. and subsp. spontaneum Brezh.) were analyzed using SDS-PAGE technique. Electrophoreograms and denzitograms of total, soluble and non-soluble proteins of 31 different samples have showed quantitative and qualitative differences. Qualitative differences in electrophoregrams of total seed proteins refer to protein fragments in zone A (114 kDa, 83 kDa and 65 kDa) and protein fragment in zone C (17 kDa). Qualitative differences in electrophoregrams of soluble seed proteins refer to protein fragment in zone A (94 kDa). Qualitative differences in electrophoregrams of nonsoluble seed proteins refer to protein fragments with molecular weights of: 210 kDa, 85 kDa, 67 kDa and 26 kDa.Semensko proteinske profile paradajza iz podvidova (subsp. cultum Brezh., subsp. subspontaneum Brezh. i subsp. spontaneum Brezh.) bili su analizirani SDS-PAGE tehnikom. Elektroforeograme i denzitograme ukupnih, rastvorljivih i nerastvorjivih semenskih proteina kod 31 razlicitog uzorka pokazale su kvantitativne i kvalitativne razlike. Kvalitativne razlike u elektroforeogramima ukupnih semenskih proteina odnose se na proteinske fragmente u zoni A (114 kDa, 83 kDa and 65 kDa) i proteinski fragmenat u zoni C (17 kDa). Kvaltativne razlike u elektroforeogramima rastvorljivih semenskih proteina odnose se na proteinski fragmenat u zoni A (94 kDa). Kvalitativne razlike u elektroforeogramima nerastvorljivih semenskih protina odnose se na proteinske fragmente sa molekularnom masom: 210 kDa, 85 kDa, 67 kDa i 26 kDa.