Histomorphometry of epithelial structures of the mare s endometrium

Abstract

The aim of this study was to analyze through histomorphometry, epithelial structures of the mare’s endometrium classified according to Kenney and Doig (1986). Histomorphometric analysis of the luminal and glandular epithelial cell heights, glandu- lar lumen diameter, glandular density, percentage of glands with apparent lumen and with intraluminal secretion, and a descriptive analysis of the endometrium was made in 65 endometrial biopsies. Samples were collected during estrus (n=30) and diestrus (n=35), fixed in Bouin’s solution, embedded in paraffin HxE and classified according to Kenney and Doig (1986) categories. Groups of IIa category obtained the greatest medium cell heights for luminal and glandular epithelium, and groups of III category obtained the smallest values, during estrus and diestrus. The medium heights of luminal epithelium, glandular epithelium and glandular lumen diameter of samples collected during estrus were greater than those collected during diestrus. The gland density was greater during diestrus than estrus. This work showed that histomorphometry could improve the consistency in biopsy evaluation by furnishing objective data.