An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals

Abstract

A method was established for the detn. of ciprofloxacin hydrochloride (CIPRO) in the blood serum and pharmaceutical samples by a combination of cloud point extn. with UV-Vis spectrophotometry. The method included the formation of Fe (III)-CIPRO complex in presence of dil. H2SO4, followed the extn. of the complex by cloud point extn. using non-ionic surfactant Triton X-114. The surfactant phase extd. was dissolved in little amt. of solvent and the CIPRO quantity measured spectrophotometrically at absorption max. of 437 nm. The effect of the exptl. factors which affect the CPE efficiency was optimized by classical method. The preconcn. and enrichment factors were found to be 40 and 143 fold resp., achieving the detection limit of 0.77 μg mL-1 and linear range of 2.5-120 μg mL-1 with correlation coeff. of 0.9984, under the optimum conditions. The av. recovery percentage of CIPRO was of 98.89 ± 0.87% and the precision in the range of 1.15-2.85%. The suggested method was applied for the detn. of CIPRO in the serum samples of different subjects orally administrated for two hours with Emicipro 500, in addn. to drug formulations like UFEXIL infusion and Ciprodar tablets. The exptl. values were agreed with the quoted values that stated by the manufacturer' companies. [on SciFinder(R)]