Effect of Physico-Chemical Parameters in the Production of Hydrolytic Enzymes from Yeast Candida Tropicalis Isolated from the Mangrove Sediments of North Kerala, India

Abstract

The extracellular enzymes produced by yeasts present in mangrove sediments were found to have wide range of biotechnological and industrial applications. Large scale production of these enzymes by bioprocess techniques needs proper optimization of culture conditions for high enzyme yield in a cost-effective manner. In the present study, we investigated the effect of different growth conditions like pH, salinity, substrate concentration and temperature on the growth and production of enzymes protease, amylase, lipase and ligninase by selected strains of Candida tropicalis. The optimum conditions for the maximum growth and enzyme production for protease, amylase, lipase and ligninase producing strains were found to be at pH 8.5-9; salinity 5-10 ppt and temperature 35 - 40°C with 2% casein, 1% starch, 1% tributyrin, 0.5% tannic acid as substrate concentrations respectively. The results suggest the use of yeast C. tropicalis from mangrove sediments as potent and promising strain for the large-scale production of hydrolytic enzymes when compared to the previous studies done on marine counter parts.