Hydrolysable Tannin Production in Oenothera tetraptera Shoot Tissue Culture

Abstract

Shoot-generating tissue cultures of Oenothera tetraptera that produce polyphenolic compounds were established. The tissues, which were subcultured on LS agar medium containing 10 µM IAA and 10 µM kinetin, produced galloylglucoses and monomeric and dimeric ellagitannins. Plants were regenerated from the tissues upon transfer from the growth regulator-containing medium to hormone-free medium. Plant regeneration from Oenothera shoots was accompanied by changes in the composition of hydrolysable tannins (galloylglucoses to oligomeric ellagitannins). © 2002, Japanese Society for Plant Cell and Molecular Biology. All rights reserved.