Comparative Study of Glutathione S-Transferase Activity of Three Human Erythrocyte Genotypes Infected With Plasmodium falciparum.

Abstract

Investigation to ascertain the levels of glutathione S-transferase (GST) activity of three human erythrocyte genotypes (HbAA, HbAS and HbSS) obtained from apparently healthy and clinically confirmedmalarious subjects/volunteers was carried out. The incubation of human erythrocytes with 1-chloro-2,4- dinitrobenzene (CDNB) resulted in almost quantitative conjugation of glutathione (GSH) to form S-(2,4-dinitrophenyl) glutathione. The reaction formed the basis for the spectrophotometric determination of GST activity. The levels of GST activity of the red cell genotypes was in the order of  bAA0.05) affect erythrocyte GST activity of non-malarious subjects/volunteers. The study suggests that GST activity evaluation might be a reliable biochemical marker and possess  promising rational for diagnostic potential in malaria