Molecular detection of some virulence genes in stahpylococcus aureus isolated from different human clinical specimens

Abstract

Staphylococcus aureus is a significant bacteria in numerous simple and more severe diseases of humans because it possesses many virulence factors which controlled by various genes that promote invention of the host cells. A total of Ninety isolates of Staphylococcus aureus obtained from various human clinical specimens distributed as urine samples (30/90) (33.3%), wound swabs (28/90) (31.1%), blood samples (24/90) (26.7%) and sputum swabs (8/90) (8.9%). The clinical specimens were submitted to diagnostic microbiology laboratories of Al-Hillah Teaching Hospital,Babylon maternity and children’s Hospital and AL-Mahaweel General Hospital during the interval from April to September 2019, in Babylon Governorate, Iraq. The multiplex PCR assay was achieved to identify Four selected virulence genes such as (sea, seb, eta and tst) in S. aureus strains by using specific primers and depending from the sizes of the products PCR amplicons. Each 90 isolates of S. aureus in human clinical specimens were examined. The results revealed that, the most frequent gene is sea 74 (82.2%), followed by seb gene 71 (78.9%), test gene 44 (48.9%) and eta gene 32 (35.6%) was reported as the least frequent detected gene. Furthermore, it was found that, the predominance toxin genes represent the highest rate in S. aureus strains isolated from wound swabs 85 (38.5%), followed by blood samples 69 (31.2%), urine samples 54 (24.4%) and finally the sputum swabs 13 (5.9%).