Formulation and evaluation of cream prepared from Croton sparsiflorus morong and their wound healing activity

Abstract

Topical application of cream prepared from the chloroform extract of Croton sparsiflorus Morong were formulated and evaluated for its efficacy and safety. Creams were prepared at different concentrations i.e. 5%, 7.5% and 10% (w/w) by fusion method using different excipients. These topical formulations were tested for pH, viscosity, spreadability and drug contents uniformity. The stability study was carried out at 4, 25 and 37 0C. The drug content uniformity of creams was found within the range of 98.09 to 98.21%. All the formulations were evaluated for its acute skin irritancy, wound healing activity in Swiss Albino rats. These formulations did not produce any skin irritation for about a week when applied over the skin. Comparative studies showed that the viscosity of the formulations increases, spreadability decreases and vice versa. From the stability studies, creams showed no changes in properties after keeping at different temperatures for 90 days. All the formulations and the normal chloroform extracts of C. sparsiflorus Morong showed significant (P<0.001) wound healing activity by excision wound model and comparable with that of the reference standards and control bases. The measurement of the wound areas were taken on 3rd, 6th, 9th, 12th, 15th and 18th days and the percentages of wound closures were calculated.