Determination of the specific absorptivity coefficient of the recombinant human epidermal growth factor

Abstract

The specific absorptivity coefficient of the recombinant human epidermal growth factor (EGFhr) was determined by a methodology that uses the technique of amino acid analysis as a method of protein quantification. Bovine serum albumin (BSA) was used as a model protein for the evaluation of the execution of the methodology. It was demonstrated by statistical analysis of the results that the coefficient of specific absorptivity of the model protein coincides with high precision with the value reported for this protein in the literature. The methodology implemented was applied to EGFhr that contains two protein species of 51 and 52 amino acids. The value of the coefficient of specific absorptivity of the two species does not present differences and can be considered as a single value, which is very useful in the productive and development context of a biopharmaceutical product.