Structure of a drug-induced DNA T-bulge: Implications for DNA frameshift mutations

Abstract

A bulged conformation in which the T3 base is flipped-out of the helical stack of the novel DNA hairpin H2 allowing complementary pairing downstream of the intercalation site (see picture) is stabilized by binding of the drug nogalamycin. This finding was readily confirmed by detailed analysis of the chemical shift and NOE data.