Genome Editing with CRISPR-Cas: An Overview

Abstract

The understanding and application of clustered regularly interspaced short palindromic repeat (CRISPR) systems and CRISPR-associated (Cas) nucleases have helped genome editing become a standard laboratory technique. CRISPR-Cas nucleases are RNA-programmed DNA-cutting enzymes that facilitate the introduction of intentional sequence changes into the genomes of experimental cells and organisms. This overview provides a background for genome editing and CRISPR-Cas nucleases, as well as an overview of the technology used to assess the outcomes of genome editing. © 2019 The Authors.