DNA barcoding markers and next generation sequence analysis of Rbcl in intraspecies genetic variations of three varieties of Cssissus quadrangularis L.

Abstract

Cissus quadrangularis L. is a well-known plant used in Ayurveda. It is also called Asthisamharaka. The etymology of its name indicates its use in the healing of the fractured bone. It is a widely growing plant belonging to the family of vitaceae. C. quadrangularis (Family: Vitaceae) is commonly distributed throughout the hotter parts of India and Sri Lanka. C. quadrangularis is mentioned in classical literature like Bhava Prakash and Chakra Dutta. The present study design to compare three different varieties of C. quadrangularis which includes the species of quadrangular, round and flat types are used to study DNA barcoding marker and phylogenetic analysis. Whole-genome was isolated from the stem parts of three varieties of C. quadrangularis; quadrangular, round and flat. The PCR amplification of DNA barcoding markers (rbcl, mat k and ITS) was analyzed by DNA electrophoresis. The DNA barcoding markers and next-generation sequencing are able to identify the intraspecies genetic variations among these closely related plant varieties of C. quadrangularis of quadrangular, round and flat.