Anticancer and Antioxidant Activity by Secondary Metabolites of Aspergillus fumigatus

Abstract

Due to the challenge faced scientists to produce the natural product as an anticancer agent from cheap sources and in endeavor to ameliorate the rate of anticancer agents, the employment of fungal metabolite in creating a cordial proper process was prerequisite. In the present study, fungal metabolites are detected by thin layer chromatography. The fungal producer strain was isolated from the rhizosphere region of old cultivated soil. One fungal isolate out of bioassay ten isolates was showed to have the most potent anticancer and antioxidant activity; this fungal isolate was identified as belonging to Aspergillus fumigatus (A. fumigatus). Fungal extract of A. fumigatus showed an antioxidant activity using Diphenyl-1-picrylhydrazyl (DPPH) scavenging % at 94.5±0.70 with IC50 at 5μg/ mL. To elucidate chemical analysis of the different bioactive compounds; A. Fumigatus metabolites extract was subjected to instrumental analysis such as GC. Mass. The A. fumigatus metabolite showed a promising anticancer activity. Inhibitory activity against Hepatocellular carcinoma cells was detected under these experimental conditions with IC50= 113 ± 3.7 µg/ml. This property can be further used to formulate new age drugs.