A method for linking yeast artificial chromosomes

Abstract

A method for linking any standard yeast artificial chromosomes (YAC) is described. YACs are introduced into the same cell and joined by mitotic recombination between the vector arms and the homologous sequence in a linking vector; several YACs can be recombined sequentially. The linking vectors also contain the β-galactosidase gene as an expression reporter in mammalian cells.