Microsatellite primers in luohanguo (Siraitia Grosvenorii, Cucurbitaceae), an economically important plant species

Abstract

Premise of the study: Microsatellite markers were developed in an economically important plant, Siraitia grosvenorii, to evaluate its genetic diversity. Methods and Results: Using the combined biotin capture method, 15 microsatellite primer sets were isolated and characterized. All of these markers showed polymorphism, and the number of alleles per locus ranged from two to 12 across 98 individuals from cultivars and a wild population. The observed and expected heterozygosities ranged from 0.000 to 0.905 and from 0.000 to 0.845, respectively. Conclusions: These markers will facilitate the breeding and further study of the genetic diversity of S. grosvenorii. © 2011 Botanical Society of America.