High-performance pipeline for MutMap and QTL-seq

Abstract

Bulked segregant analysis implemented in MutMap and QTL-seq is a powerful and efficient method to identify loci contributing to important phenotypic traits. However, the previous pipelines were not user-friendly to install and run. Here, we describe new pipelines for MutMap and QTL-seq. These updated pipelines are approximately 5-8 times faster than the previous pipeline, are easier for novice users to use, and can be easily installed through bioconda with all dependencies. Availability. The new pipelines of MutMap and QTL-seq are written in Python and can be installed via bioconda. The source code and manuals are available on- line (MutMap: https://github.com/YuSugihara/MutMap, QTL-seq: https://github.com/ YuSugihara/QTL-seq).