Effect of Response Surface Methodology-Optimized Ultrasound-Assisted Pretreatment Extraction on the Composition of Essential Oil Released From Tribute citrus Peels

Abstract

The traditional hydrodistillation (HD) and ultrasound-assisted pretreatment extraction (UAPE) methods were proposed to obtain essential oil (EO) from Tribute citrus (TC) peels. The Box-Behnken design was employed to optimize the HD and UAPE procedures. Moreover, gas chromatography-mass spectrometry (GC-MS) and electronic nose (E-nose) were applied to identify the discrepancy of the extraction methods. The yield of EO extracted by UAPE (114.02 mg/g) was significantly higher than that by HD (85.67 mg/g) (p < 0.01) undergoing 40 min short time-consuming UPAE. A total of 28 compounds were extracted from the TC peels as terpenes were the predominant components. d-Limonene was the most vital compound in the T. citrus essential oil (TCEO), accounting for 86.38% of the total volatile concentration in HD and 86.75% in UAPE, respectively, followed by α-pinene, sabinene, γ-myrcene, and β-phellandrene. The chart of radar and graphic of the principal component analysis by E-nose displayed no significance, which was similar to the GC-MS results. This study demonstrated that UAPE is an efficient and short time-consuming method for TCEO extraction, which provides a promising method for the separation of EO from aromatic plant materials.