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Determination of 2-keto-3-deoxyoctulosonic acid (KDO) with high- performance anion-exchange chromatography (HPAEC): Survey of stability of KDO and optimal hydrolytic conditions

Analytical Biochemistry (1997) 245(1) 97-101
DOI: 10.1006/abio.1996.9951
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Abstract

A new method for 2-keto-3-deoxy-octulosonic acid (KDO) determination using high-performance anion-exchange chromatography was developed. KDO is well separated from Neu5Ac, and the response was linear from 20 pmol to 5 nmol. The method was used to examine the stability of KDO under various hydrolytic conditions and to survey optimal hydrolytic conditions for release of KDO.

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Kiang, J., Szu, S. C., Wang, L. X., Tang, M., & Lee, Y. C. (1997). Determination of 2-keto-3-deoxyoctulosonic acid (KDO) with high- performance anion-exchange chromatography (HPAEC): Survey of stability of KDO and optimal hydrolytic conditions. Analytical Biochemistry, 245(1), 97–101. https://doi.org/10.1006/abio.1996.9951

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