Structure and Anticoagulant Activity of Sulfated Galactans

Abstract

Sulfated fucans are among the most widely studied of all the sulfated polysaccharides of non-mammalian ori- gin that exhibit biological activities in mammalian sys- tems. Examples of these polysaccharides extracted from echinoderms have simple structures, composed of oligo- saccharide repeating units within which the residues differ by specific patterns of sulfation among different species. In contrast the algal fucans may have some regular repeating structure but are clearly more heter- ogeneous when compared with the echinoderm fucans. The structures of the sulfated fucans from brown algae also vary from species to species. We compared the an- ticoagulant activity of the regular and repetitive fucans from echinoderms with that of the more heterogeneous fucans from three species of brown algae. Our results indicate that different structural features determine not only the anticoagulant potency of the sulfated fucans but also the mechanism by which they exert this activ- ity. Thus, the branched fucans from brown algae are direct inhibitors of thrombin, whereas the linear fucans from echinoderms require the presence of antithrombin or heparin cofactor II for inhibition of thrombin, as reported for mammalian glycosaminoglycans. The lin- ear sulfated fucans from echinoderms have an anticoag- ulant action resembling that of mammalian dermatan sulfate and a modest action through antithrombin. A single difference of one sulfate ester per tetrasaccharide repeating unit modifies the anticoagulant activity of the polysaccharide markedly. Possibly the spatial arrange- ments of sulfate esters in the repeating tetrasaccharide unit of the echinoderm fucan mimics the site in derma- tan sulfate with high affinity for heparin cofactor II.