Double-label autoradiography for comparison of complex protein mixtures after gel electrophoresis

Abstract

Comparison of complex protein mixtures by high-resolution, two-dimensional polyacrylamide gel electrophoresis is simplified by coelectrophoresis of 3H-labeled and 14C-labeled preparations, followed by two-step autoradiography. Both qualitative and quantitative comparisons are possible. Applications to genetics, molecular evolution, and metabolism are described. © 1979.