Organogenesis and somatic embryogenesis from young flower buds of agapanthus africanus hoffmanns

Abstract

Adventitious shoots and roots were directly formed from young flower bud expiants of Agapanthus africanus on Murashige and Skoog (MS) medium containing 30 g·L-1 sucrose, 2 g-L-1 gellan gum, and kinetin or thidiazuron at 1 mg-L-1 in combination with 1 mg-L-1 NAA. Friable calli with a high rate of plant regeneration were also induced by culturing the expiants on medium containing NAA, picloram or 2, 4-D at concentrations varying from 1 to 10 mg-L-41, especially at low concentrations of picloram or 2, 4-D. Shoots were readily induced from these calli when transferred onto plant growth regulat or-free or 1 mg·Lr-1 zeatincontaining MS medium. Nodular compact calli were also formed with friable calli on medium supplemented with 2, 4-D or picloram. The compact calli induced with 1 mg-L-1 picloram developed into somatic embryos. In both shoot and embryo regeneration systems, complete plantlets were obtained when they were transferred onto a medium containing no plant growth regulator.