Polyphenol Measurement and Antioxidant Activity of Miracle Fruit

Abstract

Miracle fruit has unique protein which can make sour flavors to sweet, and the bright red skin might be a natural pigment. After the harvest of miracle fruit, removal of fruit skin, fruit meat, the fruit seeds become natural side product using for biofertilizer. Recently, a growing research reports focus on the investigation of fruit seeds for screening potential antioxidants, such as steroids, phenols, and flavonoids as well. In this research, we verity miracle fruit seed is great multi-antioxidant source by different extraction solution. 3μg (GAE)/mg total phenolic and 3.1μg (RUE)/mg flavonoid content was measured when the miracle fruit was extracted by acetic acid buffer with 20mM at pH6.0 and pure ethanol. The antioxidation ability of DPPH and ABTS free radical scavenging was over 70%, chelating effect of ferrous ions reached 80%. Index Terms-Miracle fruit, fruit seed, total phenolics, antioxidant activity.