Monoclonal antibodies to human IgM

Abstract

Availability of specific antihuman IgM antibodies is very important for developing diagnostic kits for infectious diseases that are highly prevalent in Indonesia. In line with such idea, an attempt to construct hybridoma cells producing antihuman IgM antibodies is done by fusing NS-1 myeloma cell line with immunized mouse spleen cells. Hybridomas producing anti human IgM antibodies have been obtained and antibodies produced by several exhaustedly cloned hybridomas has been characterized. Those antibodies are not reactive to human IgG, IgA nor IgG and IgA-deprived human serum and belong to IgG2a subclass.