Microfluidic chemotaxis platform for differentiating the roles of soluble and bound amyloid-β on microglial accumulation

Abstract

Progressive microglial accumulation at amyloid-β (Aβ) plaques is a well-established signature of the pathology of Alzheimer's disease, but how and why microglia accumulate in the vicinity of Aβ plaques is unknown. To understand the distinct roles of Aβ on microglial accumulation, we quantified microglial responses to week-long lasting gradients of soluble Aβ and patterns of surface-bound Aβ in microfluidic chemotaxis platforms. We found that human microglia chemotaxis in gradients of soluble Aβ 42 was most effective at two distinct concentrations of 23 pg.mL-1 and 23 ng.mL-1 Aβ 42 in monomers and oligomers. We uncovered that while the chemotaxis at higher Aβ concentrations was exclusively due to Aβ gradients, chemotaxis at lower concentrations was enhanced by Aβ-induced microglial production of MCP-1. Microglial migration was inhibited by surface-bound Aβ 42 in oligomers and fibrils above 45 pg.mm-2. Better understanding of microglial migration can provide insights into the pathophysiology of senile plaques in AD.