PRODUCTION OF VITAMIN B12 BY Propionibacterium Freudenreichii AND Bacillus megaterium

Abstract

Effect of some nutritional factors on growth and vitamin B12 production by Propionibacterium freudenreichii subsp. freudenreichii CCM 1857, and Propionibacterium freudenreichii subsp. shermanii P1NRC, Bacillus megaterium DSM2894, and Bacillus megaterium 1066 was investigated. Results indicated that sodium lactate was the best carbon source for Propionibacterium spp., while glucose was the best carbon source for B. megaterium. Yeast extract plus ammonium sulphate was superior nitrogen source for Propionibacterium spp., and yeast extract was the best nitrogen source for B. megaterium. Use of combination of some elements (CoCl2 + CuSO4 + MnSO4 + ZnSO4 + MgSO4 + FeSO4) enhanced growth of Propionibacterium spp. and B. megaterium. Cobalt concentration 10mg l -1 was favor for Propionibacterium spp. however Cobalt concentration 15mg l -1 was favor for B. megaterium. High test molasses and corn meal were found to be the best agriculture by-products for Propionibacterium freudenreichii subsp. freudenreichii CCM 1857 & Bacillus megaterium DSM 2894 after being added to the basal medium.