Improved Micropropagation in Hybanthus enneaspermus L. Muell

Abstract

Multiple shoots were induced on the cut ends of the leaf of Hybanthus enneaspermus on MS medium fortified with dif-ferent concentration of cytokinins (KI and BAP) and in combination of KI and BAP. To enhance the multiple shoots induction, different types of auxins were used. IAA induced a maximum of 52.3 shoots per explants in combination with KI + BAP. The regenerated shoots show two different morphological leaves. Shoots are 100% rooted on half-strength MS medium supplemented with 2.46 µM·l –1 IBA. Rooted plantlets were successfully transferred to garden after acclimatization and they appeared morphologically normal.