Development and validation of a high performance liquid chromatographic method for determination of etoposide in biodegradable polymeric implants

Abstract

A method using HPLC-UV was developed and validated for the determination of etoposide incorporated into polycaprolactone implants. The method was carried out in isocratic mode using a C18 column (250 x 4.6 mm; 5 ?m), at 25°C, with acetonitrile and acetic acid 4% (70:30) as mobile phase, a flow rate of 2 mL/min, and UV detection at 285 nm. The method was linear (r2 > 0.99) over the range of 5 to 65 ?g/mL, precise (RSD < 5%), accurate (recovery of 98.7%), robust, selective regarding excipient of the sample, and had a quantitation limit equal to 1.76 ?g/mL. The validated method can be successfully employed for routine quality control analyses.