Fitness costs associated with laboratory induced resistance to chlorpyrifos in Spodoptera litura

Abstract

Spodoptera litura (Fabricius) is a major polyphagous pest of global relevance due to the damage it causes to various crops. Chlorpyrifos (CPF) is generally used by farmers to manage S. litura, however, its widespread use has resulted in the development of insecticide resistance. Therefore, in the present study, a population of S. litura was exposed to CPF for eight generations under laboratory conditions, resulting in a 2.81-fold resistance ratio compared with that of the unselected laboratory population (Unsel-Lab). The exposure of Unsel-Lab and CPF-Sel populations to their respective lethal and sublethal concentrations reduced larval survival, adult emergence, and prolonged development period, and induced morphological deformities in adults. The reproductive and demographic parameters were also significantly lowered in the treated larvae of both populations at higher concentrations. Moreover, hormetic effects on fecundity, next-generation larvae, the net reproductive rate (R0), and relative fitness (Rf) were observed at lower sublethal concentrations of CPF, specifically at the LC5 of Unsel-Lab and the LC10 of the CPF-Sel population. Sublethal exposure to CPF negatively affected the biological and demographic parameters in both populations, although the impact was more prominent in the CPF-Sel population. The relative fitness of the CPF-Sel was also greatly reduced at the LC50 (0.28) compared to that of the Unsel-Lab population. However, only a marginal trade-off of insecticide resistance evolution was observed in the CPF-Sel population in the absence of insecticide selection pressure. These results provide useful information for devising improved pest management strategies for CPF resistance in S. litura.