Misidentification of a rare species, cryptococcus laurentii, by commonly used commercial biochemical methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry systems: Challenges for clinical mycology laboratories

Meng Xiao; Xin Fan; Xin Xin Chen; He Wang; Li Zhang; Zhi Peng Xu; Timothy Kudinha; Fanrong Kong; Ying Chun Xu

Journal ArticleOPEN ACCESS

Misidentification of a rare species, cryptococcus laurentii, by commonly used commercial biochemical methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry systems: Challenges for clinical mycology laboratories

Journal of Clinical Microbiology (2016) 54(1) 226-229

DOI: 10.1128/JCM.02830-15

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Abstract

Forty-two putative Cryptococcus laurentii isolates identified by the Vitek 2 system were collected in China. The gold standard, internal transcribed spacer (ITS) sequencing, confirmed that only two isolates were genuine C. laurentii. Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry was able to identify the C. laurentii isolates with an expanded custom database.

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Xiao, M., Fan, X., Chen, X. X., Wang, H., Zhang, L., Xu, Z. P., … Xu, Y. C. (2016). Misidentification of a rare species, cryptococcus laurentii, by commonly used commercial biochemical methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry systems: Challenges for clinical mycology laboratories. Journal of Clinical Microbiology, 54(1), 226–229. https://doi.org/10.1128/JCM.02830-15

Misidentification of a rare species, cryptococcus laurentii, by commonly used commercial biochemical methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry systems: Challenges for clinical mycology laboratories

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