Soybean oil fat emulsion prevents cytochrome P450 mRNA down-regulation induced by fat-free overdose total parenteral nutrition in infant rats

Abstract

Changes in the mRNA expression of hepatic cytochrome P450 (CYP) isoenzymes associated with overdose of fat-free or fat-containing total parenteral nutrition (TPN) were investigated in infant rats. Three-week-old male Sprague-Dawley rats were divided into three groups: group 1 received an oral diet, group 2 received TPN without fat, and group 3 received TPN with 20% of calories from fat (soybean oil emulsion). After TPN administration for 4d, serum aspartate aminotransferase (AST) levels in group 2 were significantly increased (p<0.01) compared with the other groups. The mRNA expression of hepatic CYP isoenzymes in group 2 decreased to 0.76 to 31% of that in group 1 (p<0.01), but that in group 3 was maintained at 32 to 84% of that in group 1. These results indicate the importance of including fat in TPN regimens to prevent not only hepatic dysfunction but also mRNA down-regulation of liver CYP isoenzymes. © 2005 Pharmaceutical Society of Japan.