An Integrated Analysis of Metabolomics and Transcriptomics Reveals Significant Differences in Floral Scents and Related Gene Expression between Two Varieties of Dendrobium loddigesii

Abstract

Flower fragrance is one of the traits that holds important economical values in flowering plants. Extensive attention has converged on fragrance preservation in flower cultivation and breeding. Dendrobium loddigesii is an important species for cultivating aromatic Dendrobium orchid varieties for the long term due to its fragrance. Few studies focus on exploring related genes responsible for the aroma components in D. loddigesii. We analyzed flowers from two aromatic D. loddigesii varieties using high-throughput RNA sequencing and gas chromatography-mass spectrometry (GC-MS). The metabolomics results showed that the main volatile compounds responsible for the aroma formation of D. loddigesii were terpenes, especially monoterpenes. The de novo transcriptome assembly comprised 175,089 unigenes, and 24,570 unigenes of the genes were identified as differential expressed genes (DEGs) between the two varieties. Among these DEGs, 525 genes were mapped into seven pathways that related to the floral scent synthesis. Seventeen key genes were significantly correlated with volatile aroma metabolites, including geraniol, α-pinene, eugenol, and (Z)-3-hexenal. These results provide references for understanding the aroma biosynthesis and perfume formulations of D. loddigesii.