Effect of high-temperature extracted plant material fume against southern cowpea weevil (Callosobruchus chinensis L.) (Coleoptera: Bruchidae) as a non-chemical novel fumigation technique

Abstract

Background: Fumes from high-temperature heated plant leaves containing volatile phytochemicals generated from Lantana camara, Cinnamomum zeylanicum, Azadirachta indica and Ocimum sanctum were tested for their insecticidal activity against adult southern cowpea weevil (Callosobruchus chinensis L.) and their F1 progeny production/emergence. Volatile phytochemicals containing fume was generated using a flameless dry heat extraction method similar to pyrolysis combustion without air supplement at 180 ± 5 °C. Insect mortalities were assessed up to 72 h after exposure to the different treatments of fumigation by plant-fume and control. Results: All volatile plant-fume samples contained average of 16.3 ± 1.5% O2 and 5.8 ± 0.5% CO2 in the test jars. The F1 progeny emergence was estimated 30 days after treatment. After 36 h of exposure, L. camara showed the highest toxicity against C. chinensis, followed by O. sanctum, A. indica and C. zeylanicum, with LT50 values of 7.3, 9.4, 14.7 and 20.6 h, respectively. The volatile phytochemical containing plant-fume generated by A. indica and C. zeylanicum produced LT99 values that were not significantly different (P > 0.05) from each other. The F1 adult emergence from treated mungbean (Vigna radiata L.) samples was significantly inhibited by L. camara and A. indica volatile plant-fume compared to C. zeylanicum fume. However, plant-fume generated from all four plants exhibited effective direct toxicity and F1 progeny inhibition of more than 86%. Conclusion: From the study, it can be concluded that volatile plant-fume treatment was highly lethal to C. chinensis and significantly reduced F1 progeny emergence. Therefore, phytochemicals obtained from thermal extraction technique can be used as an alternative technique to chemical fumigation of stored mungbean.[Figure not available: see fulltext.].