Effect of exogenous gibberellin on endogenous hormone and ginkgolide content in Ginkgo leaves

Abstract

Ginkgolide is one of the important secondary metabolites of Ginkgo biloba. The synthesis of plant secondary metabolites is influenced by exogenous phytohormones. In this study, ten-year-old ginkgo grafted seedlings were used as the test material, and the effects of 0, 2, 4, 6, 8, and 10 mmol L-1 gibberellin (GA3) on endogenous indole acetic acid (IAA), abscisic acid (ABA), GA3, and ginkgolide contents in ginkgo leaves were investigated. Results showed that exogenous GA3 treatment inhibited endogenous GA3, changed the contents of endogenous IAA and ABA in ginkgo leaves, and affected the accumulation of secondary metabolite ginkgolides. Among the given concentrations, the 4 mmol L-1 GA3 treatment could remarkably increase ginkgolide content. We found that the best harvesting period of ginkgo leaves were in late August and late September, in which the content of ginkgolides was the highest.