The Effectiveness of Consuming Liberica coffee Pulp Yoghurt on Hypercholesterolemia Induced Male Sprague Dawley rats

Abstract

Hypercholesterolemia is often considered a major cause of death globally, including in Malaysia, resulting in fatalities due to heart attack or stroke. Recent research indicates that consuming coffee may lower the risk of heart failure and stroke by reducing cholesterol level. The present study investigated the efficacy of consuming Liberica coffee pulp yoghurt in female rats with hypercholesterolemia. 30 rats were divided into six different groups: a control group (GC), negative control groups (NC), a positive control group treated with simvastatin (20 mg/kg) (PC), a low dosage treatment group (8.6 mg/kg) (T1), and a high dosage treatment group (25.7 mg/kg) (T2). All rats were fed a high-cholesterol diet and received treatment daily for 8 weeks. At the end of week 8, all rats were culled. The blood and organs were obtained for biochemistry tests. The results showed that the body and organ weights of GC, PC, T1 and T2 groups show less compared to NC group. The lipid profile shows a significant (P <0.05) increase in NC group compared to other groups. Total cholesterol (TC), triglycerides (TG) and low-density lipoprotein (LDL) levels in NC group show higher among all the groups. T2 group shows significantly (P <0.05) the lowest in TC, TG and LDL levels. Liver and kidney profiles showed no significant changes. The liver histology of the NC groups showed the presence of ballooning cells compared to the other groups. Visceral fat also showed significant hyperplasia in NC group. Overall, this study demonstrated the effectiveness of L.coffee pulp yoghurt at the highest dosage able to reduce hypercholesterolemia.