Identification the local thermophilic strains of Bacillus licheniformis by VITEK-2 system and Gyrase B gene.

Abstract

One hundred and eight of soil samples have been collected from 17 area loci in Maysan governorate during the period from September to the October  2012 ,and cultivated on lauria agar media for isolation and identification of B. licheniformis. Primary identification  was depended on colonialsmorphology, microscopicsexamination and biochemicalstests then the isolates was identified by VITEK-2 compact system Version 05.01 using BCL identification cards (BCL-ID). The results revealed  that 53(94.64%) of isolates identified as  Bacillus licheniformis which could be classified in classes  where the very good identification class with percent of identification reached to  43.39%, followed by  good , acceptable, and low discrimination , which were 30.18% ,24.52% and 1.88% respectively and 3(5.35%) as unidentified organism. The isolates were identified using the PCR technique for detection of gyrase B gene clarify that 56(100%) isolates of B. licheniformis with gyrase B gene that  encodes the subunit B protein of DNA gyrase.