Ethanopharmacological study of the aqueous, chloroform, ethanol leaves extracts and latex of Calotropis procera in diabetic rats

Abstract

Abstract— Introduction: Calotropis procera is a member of the plant family Asclepiadaceae, a shrub about 6m high and is widely distributed in the kingdom of Saudi Arabia. This study aimed to show some medicinal potentials and biological ac- tivity of Calotropis procera and to discover new natural, safe and effective materials available in the Saudi Arabia environ- ment. Marerial and Methods: The leaves extracts and latex of Calotropis procera (C. procera) were investigated for its an- ti-hyperglycemic effect in Male Wister Albino rats. Diabetes was induced by administration of single dose of streptozotocin (STZ, 50 mg/kg, I.P). Fourty two male albino rats, weighting 150-200 gm divided into seven groups, each consisted of 6 rats as follows: Group I : Normal control, Group II: Diabetic control, Group III: Diabetic rats given Glibenclamide 600 μg/kg, Group IV: Diabetic rats given aqueous leaves extracts C. procera 200mg/kg b. wt, Group V: Diabetic rats given chlo- roform leaves extracts C. procera 200mg/kg b. wt, Group VI: Diabetic rats given ethanol leaves extracts C. procera 200mg/kg b. wt, Group VII: Diabetic rats given latex of C. procera 200mg/kg b. wt.The leaves extracts and latex of Calotro- pisprocera were administered as single dose per day to diabetes-induced rats for a period of 15 days.The effect of C. proce- raon blood glucose level was measured in the diabetic rats. Serum lipid profile (Total cholesterol, triglycerides, low densi- ty, and high density lipoprotein) also were measured. The activities were also compared to that effect produced by a stan- dard anti-diabetic agent, glibenclamide 500μg/kg. Results and Discussion: The results showed a significant decrease in the mean level of blood glucose and serum cholesterol, Triglycrides, HDL, LDL. Calotropis procera appears to be a rich source of phytoconstituents that activate and inhence a pharmacological response of different parts of the body and this study need further studies to shows the complete properties of the plant