Selenium absorption and retention from a selenite- or selenate-fortified milk-based formula in men measured by a stable-isotope technique

Abstract

The present study was designed to determine the apparent absorption and retention of the inorganic Se compounds SeO 3 2- and SeO 4 2- , which are commonly used for Se fortification of clinical nutrition products and infant formulas. Ten healthy men were fed a milk-based formula labelled with 40 μg Se as 74 SeO 3 2- or 76 SeO 4 2- on two consecutive days using a randomised crossover design. Se stable-isotope analysis of 9 d complete collections of urine and faeces was used to calculate apparent Se absorption and retention. Se retention from 74 SeO 3 2- (41·0 (SD 8·4) %) AND FROM 76 SEO 4 2- (46·0 (sd 7·9) %) was not significantly different (P>0·05). However, Se absorption was significantly higher from SeO 4 2- than from SeO 3 2- (91·3 (sd 1·4) % v. 50·2 (sd 7·8) %, P<0·05). Urinary excretion of the administered dose was 9·2 (sd 1·8) % for 74 SeO 3 2- and 45y3 (sd 8·2) % for 76 SeO 4 2- (P<0·05). Urinary Se excretion kinetics differed significantly for the two Se compounds; 90 % of the total urinary Se was excreted after 121 h for 74 SeO 3 2- and after 40 h for 76 SeO 4 2- (P<0·05). These results suggest that although Se absorption and urinary excretion differ for SeO 3 2- and SeO 4 2- , both Se compounds are equally well retained when administered at a relatively low dose (40 μg Se). The nutritional impact of Se fortification of foods would thus be expected to be similar when SeO 4 2- or SeO 3 2- are used.