In the present study, 310 faecal samples from goats from eight different farms in Malaysia were tested for the presence of Giardia using a PCR-coupled approach. The nested PCR for SSU amplified products of the expected size (∼200 bp) from 21 of 310 (6.8%) samples. Sixteen of these 21 products could be sequenced successfully and represented six distinct sequence types. Phylogenetic analysis of the SSU sequence data using Bayesian Inference (BI) identified Giardia assemblages A, B and E. The identification of the 'zoonotic' assemblages A and B suggests that Giardia-infected goats represent a possible reservoir for human giardiasis in Malaysia. © 2012 Elsevier Ltd.
CITATION STYLE
Lim, Y. A. L., Mahdy, M. A. K., Tan, T. K., Goh, X. T., Jex, A. R., Nolan, M. J., … Gasser, R. B. (2013). First molecular characterization of Giardia duodenalis from goats in Malaysia. Molecular and Cellular Probes, 27(1), 28–31. https://doi.org/10.1016/j.mcp.2012.08.006
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