Effect of acemannan on the dentinsialophosphoprotein and dentin matrix protein 1 mRNA expressions in primary human pulpal cells

Abstract

Objective To investigate the effect of acemannan isolated from Aloe vera gel on the steady level of dentinsialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP 1) mRNAs in primary human pulpal cells. Materials and methods Cells were treated with the designated concentrations of acemannan (0.25, 0.5 and 1 mg/ml) for 24 hours. The reverse transcription-polymerase chain reaction assays were used to investigate the effects of acemannan on the steady level of DSPP and DMP 1 mRNAs.