Perhitungan jumlah bakteri di Laboratorium Mikrobiologi menggunakan pengembangan metode Spektrofotometri

Abstract

The used of spectrophotometry in this study was a fast way to count the number of bacteria in a solution. This study aims to determine the number of test bacteria whose absorbance values are equal to the McFarland 0.5 standard solution using the cup count method. This research was conducted in The research method used consisted of several stages, namely the making of media, sterilization, purification of test bacteria, rejuvenation of test bacteria, manufacture of McFarland 0.5 standard solutions, maked standard bacteria curves, making bacterial growth curves, maked test bacterial suspensions, measuring bacterial counts turbidity method, and measurement of the number of bacteria by the cup count method. The results of this study indicate that the value of the McFarland standard solution 0.5 made according to the value of the standard McFarland solution kit (Himedia) is around 0.08-0.