Detection of infectious hematopoietic necrosis virus in fish mucus.

  • LAPATRA S
  • ROHOVEC J
  • FRYER J
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Abstract

Infectious hematopoietic necrosis virus (IHNV) was detected in mucus collected from the external surface of naturally infected juvenile and adult salmonids. Virus concentrations ranged from 101.0 to 106.0 plaque forming units (PFU) /ml. Juvenile rainbow trout Oncorhynchus mykiss artificially infected with IHNV by the waterborne route had a 100% prevalence of the virus in mucus and 50% in gill tissues 24 h post-exposure. Mucus examined from individual rainbow trout had increasing IHNV concentrations, 101.5 to 103.9 PFU/ml, from 24 to 84 h postinfection. This suggested IHNV detected in mucus resulted from the normal progression of the disease and the integument may be a site of virus replication and a possible portal of entry. 供試した自然感染サケ科魚幼魚ならびに親魚粘液から伝染性造血器懐死症ウイルス(IHNV)が検出された。ウイルス濃度は10 1.5~10 6.0 PFU/mιの範囲にあった。浸漬法でIHNVに人口感染せしめたニジマスOncorhynchus mykiss幼魚の, ウイルス接触24時間後の粘液中には100%, 鰓組織中では60%にウイルスが検出された。感染後24時間から84時間には個々のニジマスの粘液中のIHNV濃度は10 1.5から10 3.9PFU/mιに増加した。以上の結果は, 病状の進行によりIHNVが粘液から検出されるようになること, そして皮膚は多分ウイルス増殖の場であり, ウイルス侵入門戸でもあることを示唆している

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APA

LAPATRA, S. E., ROHOVEC, J. S., & FRYER, J. L. (1989). Detection of infectious hematopoietic necrosis virus in fish mucus. Fish Pathology, 24(4), 197–202. https://doi.org/10.3147/jsfp.24.197

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